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KMID : 0880220090470060710
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Journal of Microbiology
2009 Volume.47 No. 6 p.710 ~ p.715
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Scopulibacillus darangshiensis gen. nov., sp. nov., Isolated from Rock
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Lee Soon-Dong
Lee Dong-Wan
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Abstract
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A novel, Gram-positive bacterium, designated DLS-06T, was isolated from scoria (volcanic ash) under rock on the peak of small mountain (300 m above the sea level; known as Darangshi Oreum) in Jeju, Republic of Korea. The cells of the isolate were aerobic, oxidase-negative, catalase-positive, endospore- forming, non-motile rods. The organism grew at 25~30°C and initial pH 6.1~9.1. A neighbour-joining tree based on 16S rRNA gene sequences showed that the organism was related to members of the family “Sporolactobacillaceae” and related taxa. The phylogenetic neighbours were Pullulanibacillus naganoensis (95.2% 16S rRNA gene sequence similarity), Tuberibacillus calidus (95.0%) and Sporolactobacillus (91.8~94.2%). Levels of 16S rRNA gene sequence similarity of the isolate to representatives of other genera were in the range of 87.2~93.7%. The organism contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The predominant menaquinone was MK-7. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, an unknown ninhydrin-positive phospholipid, three unknown phospholipids and an unknown lipid. The major fatty acids were anteiso-C15:0 and anteiso-C17:0. The G+C content of the DNA was 50.8 mol%. On the basis of the phenotypic and phylogenetic data presented in this study, this organism represents a novel genus and species in the order Bacillales, for which the name Scopulibacillus darangshiensis gen. nov., sp. nov. is proposed. The type strain is DLS-06T (=DSM 19377T =KCTC 13161T).
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KEYWORD
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Scopulibacillus darangshiensis gen. nov., sp. nov., Darangshi Oreum, 16S rRNA gene sequence
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